Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger grouper alimentary tract Epinephelus fuscoguttatus

Lactic acid bacteria (LAB) have been isolated successfully from the tiger grouper intestine. However, their genus or species have not been Epinephelus fuscoguttatus identified. Therefore, the objective of the present study was to characterize the three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) based on their phenotype and genotype. The LAB phenotype was examined by observing morphological features including cell morphology, spore production and motility. The physiological tests were performed in 6.5% NaCl at the temperatures of 10 C and 45 C, and the biochemical tests were evaluated based on the production of enzymes catalase, oxidase and arginine dehydrolase, following the Standard Analytical Profile Index, API 50 CH kit. The genotype was examined based on 16S rDNA gene sequence analysis , and the products were analyzed using the BLAST (Basic Local Alignment Search Tool) NCBI database. The three isolates (KSBU-5Da, KSBU-12C, and KSBU-9) were categorized into the genus . 16S rDNA sequence analysis Enterococcus indicated that the isolates had 99% similarity to ATCC 9790, registered in E. hirae GenBank with accession number . It was concluded that the three LAB NR_075022.1 isolates taken from the tiger grouper are . Epinephelus fuscoguttatus E. hirae Nursyirwani Nursyirwani ( ) Corresponding author: [email protected] : Conceptualization, Data Curation, Formal Analysis, Funding Acquisition, Methodology, Project Administration, Writing – Author roles: Nursyirwani N Original Draft Preparation; : Formal Analysis, Resources, Software, Visualization; : Data Curation, Formal Analysis, Asmara W Wahyuni AETH Investigation, Resources; : Data Curation, Investigation, Resources, Software, Visualization; : Data Curation, Investigation, Methodology, Triyanto T Fauzi M Writing – Original Draft Preparation; : Resources, Supervision, Validation, Visualization, Writing – Review & Editing Muchlisin ZA No competing interests were disclosed. Competing interests: Nursyirwani N, Asmara W, Wahyuni AETH How to cite this article: et al. Phenotype and genotype of lactic acid bacteria (LAB) isolated from the tiger 2017, :1984 (doi: grouper alimentary tract [version 1; referees: awaiting peer review] Epinephelus fuscoguttatus F1000Research 6 ) 10.12688/f1000research.12734.1 © 2017 Nursyirwani N . This is an open access article distributed under the terms of the , which Copyright: et al Creative Commons Attribution Licence permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This research was partially supported by Universitas Riau. Grant information: The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. 10 Nov 2017, :1984 (doi: ) First published: 6 10.12688/f1000research.12734.1 1 2 2 3 1